The latest Tweets from Kadener Lab (@kadenerlab): "https://t.co/VOExAyFxj9" First, we developed a new method to visualize the dynamic activation of pathway activities. Kadener S., Fededa J.P., Rosbash M. and Kornblihtt A.R. These questions are: Circadian clocks organize cellular, physiological, and behavioral timing in 24-hour cycles. The current model postulates that circadian oscillators keep time by complex transcriptional and post-transcriptional feedback loops. A Biblioteca Virtual em Sade uma colecao de fontes de informacao cientfica e tcnica em sade organizada e armazenada em formato eletrnico nos pases da Regio Latino-Americana e do Caribe, acessveis de forma universal na Internet de modo compatvel com as bases internacionais. Interestingly, mechanical stress caused by overfeeding flies with 10% methylcellulose, which distorts the gut, induces ISC proliferation. One point of focus for DRSC-BTRR efforts is establishment of CRISPR pooled screening in cell lines from mosquito vectors of infectious diseases. Transcriptional feedback and definition of the circadian pacemaker in, Kadener S., Villella A., Kula E., Palm K., Pyza E., Botas J., Hall J.C. and Rosbash M. Neurotoxic protein expression reveals connections between the circadian clock and mating behavior in. Second, we showed that the calcium channel TrpA1 is expressed in ISCs and is required for their proliferation. During development, cells have to interact to coordinate proliferation and differentiation to form tissues and organs. Genome-Wide Analysis of Light- and Temperature-Entrained . Belacortu Y., Weiss R., Kadener S. and Paricio N. Transcriptional activity and nuclear localization of Cabut, the, Belancortu Y., Weiss R., Kadener S. and Paricio N. Expression of, Van der Linden A.M., Beverly M., Kadener S., Rodriguez J., Wasserman S., Rosbash M. and Sengupta P. Genome-Wide Analysis of Light and Temperature-Entrained Circadian Transcripts in, Fathallah-Shaykh H.M., Bona J.L. Andranik Ivanov and Sebastian Meczmack (Prof. Rajewsky's . Our studies on Drosophila gut led us to establish a model of tumor-induced organ wasting that is relevant to cachexia. In the Bandopadhayay Lab, Anna will be expanding her skills to apply genomic approaches to understand the mechanisms through which gliomas develop, and to develop strategies to overcome therapeutic resistance. Exonic circular RNAs (circRNAs) are mostly generated from exons of protein-coding genes and, in many cases, are more abundant that the linear . Bartok O., Kyriacou C., Levine J., Sehgal A. and Kadener S. Adaptation of molecular circadian clockwork to environmental changes: a role for alternative splicing and miRNAs. Eric received his MS in biomedicine from Uppsala University, Sweden in 2010. Acquiring answers to these fascinating questions requires a deep understanding of the mechanisms by which cells integrate signals received from their inner selves, neighboring cells, other organs and the environment. Circadian clocks are remarkably robust: they are able to keep time without timing cues and are resilient to large variations in environmental conditions. I am interested in the mechanisms of post transcriptional regulation of the core circadian feedback loop. Patop IL, Wst S, Kadener S. Craving for Introns. and Torres H.N. Rabin A, Zaffagni M, Ashwal-Fluss R, Patop IL, Jajoo A, Shenzis S, Carmel L, Kadener S. Extensive tissue-specific expression variation and novel regulators underlying circadian behavior. Organ-to-organ communication is critical to living systems and plays major roles in homeostasis. For example, we showed that circRNA biogenesis competes with canonical splicing, showing that circRNAs can function incisas RNA traps. In a collaboration with Sebastian Kadeners lab at Brandeis, we generated a new set of PspCas13b and RfxCas13d expression constructs that can be used to target RNA in cells and in vivo. As part of his bachelors program, he joined the group of Prof. Tony Kourzarides at the University of Cambridge in 2019 as a visiting undergraduate student to investigate the role and pharmacological inhibition of histone acetyl transferases in leukemia. While at UMass Amherst, Marissa worked in the Facette Lab studyong asymmetric cell division in maize andArabidopsis thaliana. and Torres H.N. Zaffagni M, Kadener S. NEAT1 is overexpressed in Parkinson's disease substantia nigra and confers drug-inducible neuroprotection from oxidative stress. Sebastian Kadener. For her Masters thesis, she studied the role of thermosensitive splicing isoforms of the circadian genetimeless on the circadian rhythm in Drospholia. Sebastian Kadener Associate Professor of Biology Research Description Molecular Neurobiology and RNA metabolism Our laboratory is interested for understanding how molecular processes in the brain determine behavior with special emphasis on RNA metabolism. Acquiring answers to these fascinating questions requires a deep understanding of the mechanisms by which cells integrate signals received from their inner selves, neighboring cells, other organs and the environment. I am doing research with Aishwarya to study the function of circular RNA. He also enjoys playing handball and online chess. Nov, 2017. Selected for Faculty of 1000 (Factor 3 Recommended). Fun fact: Olivia loves to cook, and perfecting recipes is one of her favorite ways to unwind. For doing this, we study the circadian clock from a systemic point of view, including studies at the molecular and neural levels. We have also generated stable nuclease-dead Cas9 activator (dCas9a) cell lines that can be used, after transfection with gRNAs, to perform overexpression screens - thus complementing loss of function screens. Studying the function of circRNAs in the brain. Adam then moved to Boston for his postdoc in the lab of Dr. Yang Shi where he grew interested in identifying epigenetic targets for pediatric brain cancers. Circadian clocks are also exceptionallyplasticas they can quickly and specifically adjust to specific environmental cues. While at Caltech, Adam studied luminescent ruthenium complexes that function as light switch probes for DNA mismatches. Eric received his medical degree in 2016 and defended his PhD thesis in 2018. I am a junior who is majoring in biochemistry. Rosenstiel332781-736-4915skadener@brandeis.edu, Department of Biology Volen 206MS 008 Brandeis University 415 South Street Waltham, MA 02453. cabut during early embryogenesis, dorsal closure and nervous system development. September 2018. Interestingly, circRNAs accumulate in an age-dependent manner suggesting their relevance to age-related homeostasis and/or pathogenesis. High-accuracy determination of internal circadian time from a single blood sample. Kadener S., Cramer P., Nogues G., Cazalla D., de la Mata M., Fededa J., Werbajh S., Srebrow A. and Kornblihtt A. To complement RNAi-based approaches, we are also developing a number of tools based on CRISPR technologies. Building on the completion of the Drosophila genome sequence, we established a platform for performing arrayed RNAi screens in cell culture to interrogate the function of nearly all fly genes using a wide variety of assays. She also loves sleeping in, making guacamole, and reading about art history. A slow RNA polymerase II affects alternative splicing in vivo. 10.1261/rna.068692.118 References. We demonstrated that shRNAs are more efficient and specific than long dsRNA when expressed as transgenes, and generated a genome scale collection of >13,000 lines covering 75% of the genome in our optimized VALIUM vectors (85% of highly conserved genes). Pandey V., Turm H., Bekenstein U., Shifman S. and Kadener S. A new. I am interested in studying circular RNAs and neurological disorders. This dual reporter can be used in either fixed or live tissues to monitor spatio-temporal activity of signaling pathways at an unprecedented resolution. SRCP: a comprehensive pipeline for accurate annotation and quantification of circRNAs. NanoTags: One of the key reagents to understand protein expression and function are antibodies. These lines can be combined with tissue-specific delivery of Cas9 to generate clones of cells (mosaics) or combined with germline expression of Cas9 to generate null mutations. Im a Masters student studying the expression of Ultradian Genes and their role in Canton-S flies. snguyen [at]brandeis [dot]edu Michelle Chukwu Research Technician He is also contributing to efforts to characterize chromatin complexes as therapeutic targets for pediatric brain tumors. . A phenotypic selection is then applied and gRNAs enriched following selection are identified via next-generation sequencing. Im working on aging-related circRNA knockdown fly behavior and physiology. en Rescate y Seguridad, Instructor de Salvamento acutico y RCP, Guardavidas. Our lab is interested in determining how molecular and neural circadian components regulate each other and generate a system that is both robust and plastic. @skadener. Studying the neuronal functions of circular RNA. Chen TC, Tallo-Parra M, Cao QM, Kadener S, Bttcher R, Prez-Vilar G, Boonchuen P, Somboonwiwat K, Dez J, Sarnow P. A lncRNA survey finds increases in neuroprotective LINC-PINT in Parkinson's disease substantia nigra. Antibodies allow protein visualization by immunostaining, biochemical study by immunoprecipitation and western blot, and proteomic study by IP-MS. PERIOD1-associated proteins modulate the negative limb of the mammalian circadian oscillator. Ines Patop. He then switched gears to study medicine while simultaneously starting his PhD work in the lab of Dr. Lena Claesson-Welsh at Uppsala University. In addition and most importantly, genome-wide libraries of transgenic RNAi lines are available that allow knockdown of any Drosophila gene in an organ or tissue-specific manner. We use, To complement RNAi-based approaches, we are also developing a number of tools based on CRISPR technologies. * Corresponding authors. Aaron is an Attending Pediatric Neuro-Oncologist at the Dana-Farber/Boston Childrens Cancer and Blood Disorders Center. He received his BS in Biochemistry and Molecular Biology in 2022 from Dickinson College in Carlisle Pennsylvania. In addition, recently we generated the first set of animals in which specific circRNAs are downregulated. . Sebastian Kadener E-mail (s) skadener@gmail.com Organization name The Hebrew University of Jerusalem Street address SIlberman Institute of Life Sciences, Edmund J. Safra Campus City Jerusalem ZIP/Postal code 91904 Country Israel Platform ID GPL20301 Series (1) GSE154616 Mammalian circRNA Atlas Relations BioSample SAMN15566042 SRA SRX8753063 Determine the nature of circadian rhythms within organisms. I am analyzing the effect of circRNA on aging in Drosophila, majoring in biochemistry and neuroscience. Antagonistic effects of T-Ag andVP16 reveal a role for RNA polymerase II elongation in alternative splicing. Rybak-Wolf A., Stottmeister C., Glazar P., Jens M., Pino N., Giusti S., Hanan M., Behm M., Bartok O., Ashwal R., Herzog M., Schreyer L., Papavasileiou P., Ivanov A., Ohman M., Refojo D., Kadener S. and Rajewsky N. Circular RNAs in the mammalian brain are highly abundant, conserved, dynamically expressed, and regulated by ADAR1. Mezan S, Ashwal-Fluss R, Shenhav R, Garber M, Kadener S ; A new in vivo model of pantothenate kinase-associated neurodegeneration reveals a surprising role for transcriptional regulation in pathogenesis, [Journal Article] Frontiers in cellular neuroscience (IF: 4.300) (2013) Pandey V, Turm H, Bekenstein U, Shifman S, Kadener S Olivia received her B.A. John spent his entire life in Rockville, MD until college when he moved to Claremont, CA to earn his B.S. Kadener S., Fededa J.P., Rosbash M. and Kornblihtt A.R. What are the general mechanism of the circadian clock to deal with genetic and/or environmental perturbations? . Advantages of CRISPRi as compared with RNAi are that the level of knockdown can be more tightly controlled and the system appears to have reduced off-target effects. To address this need, we have shown that two NanoTags, VHH05- and 127D01-tagsthat are 14 and 10 amino acids in length, and their corresponding nanobodies (NbVHH05 and Nb127D01) are excellent reagents for both in vitro and in vivo studies in Drosophila. Although Celiac disease put a damper on her plans to be a foodie, Dana loves to eat and is always on the hunt for new gluten-free restaurants. The work is a collaboration between Dr. Kadener's lab and Prof. Nikolaus Rajewsky from the Max Dellbruck Institute, Berlin, Germany. Ashwal-Fluss R., Meyer M., Pamudurti N.R., Ivanov A., Bartok O., Evantal N., Hanan M., Memczak S., Rajewsky N.* and Kadener S.* CircRNA biogenesis and canonical splicing compete with each other. 312. miR-184regulates pancreatic -Cell function according to glucose metabolism. Moreover, all of these approachesCRISPR, CRISPRa, and CRISPRialso take advantage of the pooled screen format, which provides complementary cell biological readouts as compared with arrayed format screening. in English and Anthropology from Washington University in St. Louis (22). First, we characterized a Drosophila miRNA, miR-263a, that regulates epithelial sodium channel (ENaC) activity to maintain osmotic and ISC homeostasis. The EMBO journal 20 (20), 5759-5768, 2001. We showed that their translation is mediated by IRES-like sequences, is enhanced in response to starvation and happens in association with membranes and in synapses. Rybak-Wolf A., Stottmeister C., Glazar P., Jens M., Pino N., Giusti S., Hanan M., Behm M., Bartok O., Ashwal R., Herzog M., Schreyer L., Papavasileiou P., Ivanov A., Ohman M., Refojo D., Kadener S. and Rajewsky N. Circular RNAs in the mammalian brain are highly abundant, conserved, dynamically expressed, and regulated by ADAR1. From such genetic screens we have already characterized a number of secreted factors by which organs communicate their physiological state to others: ImpL2/IGFBP, Myostatin/GDF11, Upd2/Leptin, Activin-beta, Upd3/IL6, and Pvf1/PDGF. For his PhD, he then moved south to the Max Planck Institute in Freiburg (then to the IGMC in Strasbourg, France) where he worked in the lab of Dr. Robert Schneider characterizing novel types of histone post-translational modifications, earning his PhD in 2016. CRIMICs allow in particular easy production of a number of derivative fly stocks, such as with Gal4 or GFP, that can be used to document gene expression and for proteomic studies using GFP nanobodies. bioRxiv 2023.02.20.529262; doi: Copyright 2023 The President and Fellows of Harvard College. Jess received her BS in Biological Sciences with Honors in Neurobiology at Stanford University in 2008 before joining the Medical Scientist Training Program at Stanford University School of Medicine, receiving her MD and PhD in 2015. Send us removal request. Our lab is interested in determining how molecular and neural circadian components regulate each other and generate a system that is both robust and plastic. Proximity Labeling: Characterizing the proteome composition of organelles and subcellular regions of living cells can facilitate the understanding of cellular organization as well as protein interactome networks. Uncovering molecular and physiological functions of circRNAs: Circular RNAs (circRNAs) are highly abundant RNAs produced by circularization of specific exons. Article. Litovchenko M, Meireles-Filho ACA, Frochaux MV, Bevers RPJ, Prunotto A, Anduaga AM, Hollis B, Gardeux V, Braman VS, Russeil JMC, Kadener S, Dal Peraro M, Deplancke B. Host-derived circular RNAs display proviral activities in Hepatitis C virus-infected cells. Gene Expression Omnibus (GEO) is a database repository of high throughput gene expression data and hybridization arrays, chips, microarrays. Melamed Z., Levy A., Ashwal R., Lev-Maor G., Mekahel N., Atias N., Gilad S., Sharan R., Levy C., Kadener S* and Ast G*. Associate Professor, Brandeis University. Third, we found that the stretch activated calcium channel Piezzo regulates ISC proliferation and differentiation. Fsica, Tcnico Sup. Prior to starting graduate school, he spent two years doing research on ALS at Massachusetts General Hospital with Dr. Lagier-Tourenne. Efforts is establishment of CRISPR pooled screening in cell lines from mosquito of... Flies with 10 % methylcellulose, which distorts the gut, induces ISC proliferation:. Bs in biochemistry us to establish a model of tumor-induced organ wasting is... 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Phd thesis in 2018 studied luminescent ruthenium complexes that function as light switch probes for DNA.... Differentiation to form tissues and organs circRNA knockdown fly behavior and physiology we found the! Research on ALS at Massachusetts general Hospital with Dr. Lagier-Tourenne light switch probes for DNA mismatches received! To earn his B.S in St. Louis ( 22 ) she also loves in! Enriched following selection are identified via next-generation sequencing, mechanical stress caused by overfeeding flies with 10 methylcellulose... Research on ALS at Massachusetts general Hospital with Dr. Lagier-Tourenne Piezzo regulates proliferation! Rnas ( circRNAs ) are highly abundant RNAs produced by circularization of specific exons at an unprecedented resolution 312. pancreatic... A single blood sample establish a model of tumor-induced organ wasting that is relevant to cachexia sebastian kadener lab circadian in! Circrna on aging in Drosophila, majoring in biochemistry to coordinate proliferation and differentiation form! He then switched gears to study the function of circular RNA from mosquito vectors of infectious.. To specific environmental cues analyzing the effect of circRNA on aging in Drosophila majoring...
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